Anti-Hsp40 抗体 (ab69402)
Key features and details
- Rabbit polyclonal to Hsp40
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
- Isotype: IgG
製品の概要
-
製品名
Anti-Hsp40 antibody
Hsp40 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Hsp40 -
由来種
Rabbit -
特異性
This antibody detects a ~40 kDa protein, corresponding to the apparent molecular mass of Hsp40 on SDS-PAGE immunoblots. It does not detect DnaJ in E.coli lysates.
-
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human, Recombinant fragment
非交差種: Escherichia coli -
免疫原
Recombinant human Hsp40
-
ポジティブ・コントロール
- WB: Heat shocked HeLa cells and 3T3 cells, PC-12 cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69402の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (2) |
1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
|
特記事項 |
---|
WB
1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa). |
ターゲット情報
-
機能
Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. -
配列類似性
Contains 1 J domain. -
細胞内局在
Cytoplasm. Nucleus. Nucleus > nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock. - Information by UniProt
-
参照データベース
- Entrez Gene: 3337 Human
- Entrez Gene: 81489 Mouse
- Entrez Gene: 361384 Rat
- Omim: 604572 Human
- SwissProt: P25685 Human
- SwissProt: Q9QYJ3 Mouse
- Unigene: 515210 Human
- Unigene: 282092 Mouse
-
別名
- DnaJ (Hsp40) homolog subfmaily B member 1 antibody
- DNAJ 1 antibody
- DNAJ B1 antibody
see all
画像
-
All lanes : Anti-Hsp40 antibody (ab69402) at 1/1000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Hsp40 recombinant protein
Lane 3 : DnaJ recombinant protein
Lane 4 : Cell lysates prepared from heat shocked hela cells
Lane 5 : Cell lysates prepared from heat shocked 3T3 cells
Lane 6 : Cell lysates prepared from PC-12 cells
Predicted band size: 38 kDa
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (13)
ab69402 は 13 報の論文で使用されています。
- Gulati R et al. Phosphorylation-Mediated Activation of β-Catenin-TCF4-CEGRs/ALCDs Pathway Is an Essential Event in Development of Aggressive Hepatoblastoma. Cancers (Basel) 14:N/A (2022). PubMed: 36551548
- Capitano ML et al. Mitigating oxygen stress enhances aged mouse hematopoietic stem cell numbers and function. J Clin Invest 131:N/A (2021). PubMed: 33393491
- Zhang Y et al. Proteomic Profiling of Purified Rabies Virus Particles. Virol Sin 35:143-155 (2020). PubMed: 31429011
- Nadeem A et al. Beta-sheet-specific interactions with heat shock proteins define a mechanism of delayed tumor cell death in response to HAMLET. J Mol Biol 431:2612-2627 (2019). PubMed: 31082436
- Zhang W et al. Cellular DNAJA3, a Novel VP1-Interacting Protein, Inhibits Foot-and-Mouth Disease Virus Replication by Inducing Lysosomal Degradation of VP1 and Attenuating Its Antagonistic Role in the Beta Interferon Signaling Pathway. J Virol 93:N/A (2019). PubMed: 30996089