製品の概要

  • 製品名Anti-Hsp40 antibody [3B9.E6]
    Hsp40 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3B9.E6] to Hsp40
  • 特異性ab78437 does not cross-react with Hdj2 or Ydj1.
  • アプリケーション適用あり: WB, IP, ELISA, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant Human Hsp40

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名3B9.E6
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab78437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.
  • 配列類似性Contains 1 J domain.
  • 細胞内局在Cytoplasm. Nucleus. Nucleus > nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DnaJ (Hsp40) homolog subfmaily B member 1 antibody
    • DNAJ 1 antibody
    • DNAJ B1 antibody
    • DnaJ homolog subfamily B member 1 antibody
    • DnaJ protein homolog 1 antibody
    • DNAJ1 antibody
    • DNAJB 1 antibody
    • Dnajb1 antibody
    • DNAJB1 protein antibody
    • DNJB1_HUMAN antibody
    • HDJ 1 antibody
    • HDJ-1 antibody
    • HDJ1 antibody
    • Heat shock 40 kDa protein 1 antibody
    • Heat shock 40kD protein 1 antibody
    • Heat shock protein 40 antibody
    • Hsp 40 antibody
    • HSP40 antibody
    • HSPF 1 antibody
    • HSPF1 antibody
    • Human DnaJ protein 1 antibody
    • Radial spoke 16 homolog B antibody
    • RSPH16B antibody
    • Sis1 antibody
    see all

Anti-Hsp40 antibody [3B9.E6] 画像

  • Overlay histogram showing HeLa cells stained with ab78437 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78437, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Hsp40 antibody [3B9.E6] (ab78437) 使用論文

This product has been referenced in:
  • Jo S  et al. Neuronal hypoxia induces hsp40-mediated nuclear import of type 3 deiodinase as an adaptive mechanism to reduce cellular metabolism. J Neurosci 32:8491-500 (2012). Rat . Read more (PubMed: 22723689) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"