製品の概要

  • 製品名Anti-Hsp27 antibody [G3.1] (Biotin)
    Hsp27 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [G3.1] to Hsp27 (Biotin)
  • 標識Biotin
  • 特異性ab69556 does not exhibit reactivity with rodent Hsp25, alpha A, alpha B or beta-crystallin proteins.
  • アプリケーション適用あり: ELISA, IHC-P, WB, IP, ICC, Electron Microscopy, Functional Studiesmore details
  • 種交差性
    交差種: Human, Monkey
  • 免疫原

    Purified Hsp24 isolated from MCF-7, a human breast tumor cell line. Upon cloning and sequence analysis of the gene encoding the 24 kDa protein, nucleotide sequence comparison revealed complete identity with the small heat shock protein, Hsp27.

  • ポジティブ・コントロール
    • HeLa Heat Shocked Cell Lysate.
  • 特記事項Storage below -20°C is not recommended.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa.
IP Use at an assay dependent dilution.
ICC Use a concentration of 10 µg/ml.
Electron Microscopy 1/50.
Functional Studies Use at an assay dependent dilution.

ターゲット情報

  • 機能Involved in stress resistance and actin organization.
  • 組織特異性Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
  • 関連疾患Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
    Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
  • 配列類似性Belongs to the small heat shock protein (HSP20) family.
  • 翻訳後修飾Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
  • 細胞内局在Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Heat shock 27kDa protein antibody
    • 28 kDa heat shock protein antibody
    • CMT2F antibody
    • DKFZp586P1322 antibody
    • epididymis secretory protein Li 102 antibody
    • Estrogen regulated 24 kDa protein antibody
    • Estrogen-regulated 24 kDa protein antibody
    • Heat shock 25kDa protein 1 antibody
    • Heat shock 27 kDa protein antibody
    • Heat shock 27kD protein 1 antibody
    • Heat shock 27kDa protein 1 antibody
    • Heat shock 28kDa protein 1 antibody
    • Heat Shock Protein 27 antibody
    • Heat shock protein beta 1 antibody
    • Heat shock protein beta-1 antibody
    • heat shock protein family B (small) member 1 antibody
    • HEL-S-102 antibody
    • HMN2B antibody
    • HS.76067 antibody
    • Hsp 25 antibody
    • HSP 27 antibody
    • Hsp 28 antibody
    • Hsp B1 antibody
    • Hsp25 antibody
    • HSP27 antibody
    • Hsp28 antibody
    • HspB1 antibody
    • HSPB1_HUMAN antibody
    • SRP27 antibody
    • Stress responsive protein 27 antibody
    • Stress-responsive protein 27 antibody
    see all

Anti-Hsp27 antibody [G3.1] (Biotin) 画像

  • All lanes : Anti-Hsp27 antibody [G3.1] (Biotin) (ab69556) at 1/1000 dilution

    Lane 1 : Hsp27 recombinant protein
    Lane 2 : Cell lysates prepared from heat shock treated Hela cells
    Lane 3 : Cell lysates prepared from heat shock treated Vero cells


    Predicted band size : 23 kDa

Anti-Hsp27 antibody [G3.1] (Biotin) (ab69556) 使用論文

ab69556 has not yet been referenced specifically in any publications.

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