製品の概要

  • 製品名Anti-Hsp27 antibody [G3.1]
    Hsp27 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [G3.1] to Hsp27
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC, Electron Microscopy, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human, Fish, Monkey
  • 免疫原

    Full length native protein Human Hsp27.

  • ポジティブ・コントロール
    • HeLa and Vero cell lysate, Human Hsp27 and Mouse Hsp27 recombinant protein, Human skeletal muscle tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 27 kDa (predicted molecular weight: 23 kDa). colorimetric WB
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.
ICC Use at an assay dependent concentration.
Electron Microscopy Use at an assay dependent concentration.
Flow Cyt Use 0.1-1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Involved in stress resistance and actin organization.
  • 組織特異性Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
  • 関連疾患Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
    Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
  • 配列類似性Belongs to the small heat shock protein (HSP20) family.
  • 翻訳後修飾Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
  • 細胞内局在Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Heat shock 27kDa protein antibody
    • 28 kDa heat shock protein antibody
    • CMT2F antibody
    • DKFZp586P1322 antibody
    • epididymis secretory protein Li 102 antibody
    • Estrogen regulated 24 kDa protein antibody
    • Estrogen-regulated 24 kDa protein antibody
    • Heat shock 25kDa protein 1 antibody
    • Heat shock 27 kDa protein antibody
    • Heat shock 27kD protein 1 antibody
    • Heat shock 27kDa protein 1 antibody
    • Heat shock 28kDa protein 1 antibody
    • Heat Shock Protein 27 antibody
    • Heat shock protein beta 1 antibody
    • Heat shock protein beta-1 antibody
    • heat shock protein family B (small) member 1 antibody
    • HEL-S-102 antibody
    • HMN2B antibody
    • HS.76067 antibody
    • Hsp 25 antibody
    • HSP 27 antibody
    • Hsp 28 antibody
    • Hsp B1 antibody
    • Hsp25 antibody
    • HSP27 antibody
    • Hsp28 antibody
    • HspB1 antibody
    • HSPB1_HUMAN antibody
    • SRP27 antibody
    • Stress responsive protein 27 antibody
    • Stress-responsive protein 27 antibody
    see all

Anti-Hsp27 antibody [G3.1] 画像

  • Overlay histogram showing HeLa cells stained with ab115846 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115846, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • All lanes : Anti-Hsp27 antibody [G3.1] (ab115846) at 1/1000 dilution

    Lane 1 : MW ladder
    Lane 2 : HeLa heat shocked
    Lane 3 : Vero heat shocked
    Lane 4 : human Hsp27 recombinant protein


    Predicted band size : 23 kDa
  • Immunohistochemistry analysis of Human skeletal muscle tissue stained with ab115846 at a concentration of 10µg/ml.

Anti-Hsp27 antibody [G3.1] (ab115846) 使用論文

This product has been referenced in:
  • Vidyasagar A  et al. HSP27 is involved in the pathogenesis of kidney tubulointerstitial fibrosis. Am J Physiol Renal Physiol 295:F707-16 (2008). Read more (PubMed: 18596079) »
  • Radice S  et al. Estrogenic activity of procymidone in primary cultured rainbow trout hepatocytes (Oncorhynchus mykiss). Toxicol In Vitro 16:475-80 (2002). Read more (PubMed: 12110288) »

See all 5 Publications for this product

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Thank you for your enquiry which has been forwarded to the scientific support team. I am sorry we do not provide larger vial sizes. We can offer a discount if you purchase 5 vials or more on one order (of the same product, or different products). I...

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