製品の概要

  • 製品名Anti-Hsp27 antibody [5D7]
    Hsp27 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5D7] to Hsp27
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Purified recombinant fragment of human Hsp27 expressed in E. Coli.

  • ポジティブ・コントロール
    • WB: Hela; A549; Jurkat; A431; HEK293; HepG2 and PC-12 cell lysates. IHC-P: Brain tissues; esophageal cancer tissues.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab114067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 27 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/200 - 1/1000.

ターゲット情報

  • 機能Involved in stress resistance and actin organization.
  • 組織特異性Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.
  • 関連疾患Defects in HSPB1 are the cause of Charcot-Marie-Tooth disease type 2F (CMT2F) [MIM:606595]. CMT2F is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. CMT2F onset is between 15 and 25 years with muscle weakness and atrophy usually beginning in feet and legs (peroneal distribution). Upper limb involvement occurs later. CMT2F inheritance is autosomal dominant.
    Defects in HSPB1 are a cause of distal hereditary motor neuronopathy type 2B (HMN2B) [MIM:608634]. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective impairment of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs.
  • 配列類似性Belongs to the small heat shock protein (HSP20) family.
  • 翻訳後修飾Phosphorylated in MCF-7 cells on exposure to protein kinase C activators and heat shock.
  • 細胞内局在Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Heat shock 27kDa protein antibody
    • 28 kDa heat shock protein antibody
    • CMT2F antibody
    • DKFZp586P1322 antibody
    • epididymis secretory protein Li 102 antibody
    • Estrogen regulated 24 kDa protein antibody
    • Estrogen-regulated 24 kDa protein antibody
    • Heat shock 25kDa protein 1 antibody
    • Heat shock 27 kDa protein antibody
    • Heat shock 27kD protein 1 antibody
    • Heat shock 27kDa protein 1 antibody
    • Heat shock 28kDa protein 1 antibody
    • Heat Shock Protein 27 antibody
    • Heat shock protein beta 1 antibody
    • Heat shock protein beta-1 antibody
    • heat shock protein family B (small) member 1 antibody
    • HEL-S-102 antibody
    • HMN2B antibody
    • HS.76067 antibody
    • Hsp 25 antibody
    • HSP 27 antibody
    • Hsp 28 antibody
    • Hsp B1 antibody
    • Hsp25 antibody
    • HSP27 antibody
    • Hsp28 antibody
    • HspB1 antibody
    • HSPB1_HUMAN antibody
    • SRP27 antibody
    • Stress responsive protein 27 antibody
    • Stress-responsive protein 27 antibody
    see all

Anti-Hsp27 antibody [5D7] 画像

  • All lanes : Anti-Hsp27 antibody [5D7] (ab114067) at 1/500 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : A431 cell lysate
    Lane 5 : HEK293 cell lysate
    Lane 6 : HepG2 cell lysate
    Lane 7 : PC-12 cell lysate


    Predicted band size : 27 kDa
  • Immunofluorescence analysis of Hela cells using ab114067 at 1/200 dilution (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Immunohistochemical analysis of paraffin embedded breast cancer tissues (left) and cardiac muscle tissues (right) using ab114067 at 1/200 dilution with DAB staining.
  • Immunohistochemical analysis of paraffin embedded brain tissues (left) and esophageal cancer tissues (right) using ab114067 at 1/200 dilution with DAB staining.
  • Flow cytometric analysis of HepG2 cells using ab114067 at 1/200 dilution (green) and negative control (purple).

Anti-Hsp27 antibody [5D7] (ab114067) 使用論文

This product has been referenced in:
  • Deng H  et al. Ac-SDKP suppresses epithelial-mesenchymal transition in A549 cells via HSP27 signaling. Exp Mol Pathol 97:176-83 (2014). Human . Read more (PubMed: 24998956) »
  • Langer R  et al. Protein expression profiling in esophageal adenocarcinoma patients indicates association of heat-shock protein 27 expression and chemotherapy response. Clin Cancer Res 14:8279-87 (2008). Read more (PubMed: 19088045) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry which has been forwarded to the scientific support team. I am sorry we do not provide larger vial sizes. We can offer a discount if you purchase 5 vials or more on one order (of the same product, or different products). I...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"