Rabbit polyclonal to Hsp20
Mouse, Rat, Cow
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Hsp20.
(Peptide available as
This antibody gave a positive signal in the following whole cell lysates: HeLa; HepG2; Jurkat; JEG-3; A549.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 17 kDa).
Use a concentration of 5 µg/ml.
Belongs to the small heat shock protein (HSP20) family.
The N-terminus is blocked.
Cytoplasm. Nucleus. Translocates to nuclear foci during heat shock.
Information by UniProt
epididymis luminal protein 55 antibody
Heat shock 20 kDa like protein p20 antibody
Western blot - Anti-Hsp20 antibody (ab68977)
All lanes :
Anti-Hsp20 antibody (ab68977) at 1 µg/ml
Lane 1 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 :
Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 :
JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 5 :
A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
16 kDa (
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-Hsp20 antibody (ab68977)
ICC/IF image of ab68977 stained HeLa cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68977, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) Hek293 cells at 5µg/ml, and in 4% PFA fixed (10 min) MCF-7 cells at 5µg/ml.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"