Anti-Hsp20 抗体 (ab13491)
Key features and details
- Rabbit polyclonal to Hsp20
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Hsp20 antibody
Hsp20 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Hsp20 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Rat skeletal muscle Hsp20.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 0.268% PBS, 3% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13491の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 0.05 µg/ml. Detects a band of approximately 20 kDa.
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特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 0.05 µg/ml. Detects a band of approximately 20 kDa. |
ターゲット情報
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配列類似性
Belongs to the small heat shock protein (HSP20) family. -
翻訳後修飾
The N-terminus is blocked. -
細胞内局在
Cytoplasm. Nucleus. Translocates to nuclear foci during heat shock. - Information by UniProt
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参照データベース
- Entrez Gene: 126393 Human
- Entrez Gene: 192245 Rat
- Omim: 610695 Human
- SwissProt: O14558 Human
- SwissProt: P97541 Rat
- Unigene: 534538 Human
- Unigene: 3201 Rat
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別名
- epididymis luminal protein 55 antibody
- FLJ32389 antibody
- Heat shock 20 kDa like protein p20 antibody
see all
画像
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Anti-Hsp20 antibody (ab13491) at 1/2000 dilution + Lysates prepared from rat heart tissue
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ICC/IF image of ab13491 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13491, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (8)
ab13491 は 8 報の論文で使用されています。
- Lee B et al. Associations of Apoptotic and Anti-Apoptotic Factors with Beef Quality, Histochemical Characteristics, and Palatability of Hanwoo Longissimus thoracis Muscle. Animals (Basel) 12:N/A (2022). PubMed: 35203175
- Beuzelin D et al. Oral Delivery of miRNA With Lipidic Aminoglycoside Derivatives in the Breastfed Rat. Front Physiol 10:1037 (2019). PubMed: 31456698
- Hedegaard ER et al. Mechanisms involved in increased sensitivity to adenosine A(2A) receptor activation and hypoxia-induced vasodilatation in porcine coronary arteries. Eur J Pharmacol 723:216-26 (2014). WB . PubMed: 24309216
- Kirui DK et al. Transient mild hyperthermia induces E-selectin mediated localization of mesoporous silicon vectors in solid tumors. PLoS One 9:e86489 (2014). WB ; Human . PubMed: 24558362
- Chen A et al. Lysine deacetylase inhibition promotes relaxation of arterial tone and C-terminal acetylation of HSPB6 (Hsp20) in vascular smooth muscle cells. Physiol Rep 1:e00127 (2013). PubMed: 24400135
- Dordea AC et al. Differential vasodilation of human placental and myometrial arteries related to myofilament Ca2+-desensitization and the expression of Hsp20 but not MYPT1. Mol Hum Reprod N/A:N/A (2013). Human . PubMed: 23775458
- Zhou YC et al. Effects of buyang huanwu decoction on ventricular remodeling and differential protein profile in a rat model of myocardial infarction. Evid Based Complement Alternat Med 2012:385247 (2012). IHC-P ; Rat . PubMed: 23049607
- Fontaine JM et al. Interactions of HSP22 (HSPB8) with HSP20, alphaB-crystallin, and HSPB3. Biochem Biophys Res Commun 337:1006-11 (2005). WB ; African green monkey . PubMed: 16225851