製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Hsp104
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Hamster, Saccharomyces cerevisiae
    非交差種: Drosophila melanogaster, Plants, Escherichia coli
  • 免疫原

    Synthetic peptide corresponding to Saccharomyces cerevisiae Hsp104 aa 894-908 (C terminal).
    Sequence:

    DDDNEDSMEIDDDLD

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium azide
  • 精製度
    Whole antiserum
  • 一次抗体 備考
    Heat Shock Proteins (HSP) are expressed in response to various biological stresses, including high temperatures. There are several major families of HSPs including HSP 70, HSP 90 and HSP 100. The HSP 100 proteins generally have amino acid sequences of about 900 residues and contain two nucleotide-binding sites. Within the HSP 100 family of proteins, yeast express an ~104 kDa form which is necessary to protect cells from various stress conditions such as heat, heavy metals and ethanol, though mutation studies have shown that the protein is not required for normal growth. Yeast HSP 104 has been shown to be a ClpB protein with significant sequence homology to E. coli ClpB, particularly in the two nucleotide-binding sites.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/5000. Detects a band of approximately 100-105 kDa. 1/100 for Mammals and 1/500 for yeast.
IHC-P 1/100 - 1/200.

ターゲット情報

  • 関連性
    Hsp104 is a molecular chaperone required for stress tolerance and for maintenance of [psi(+)] prions in the budding yeast Saccharomyces cerevisiae. Hsp104 is vital to protect yeast cells against high temperature and high concentration of ethanol. It is not required for normal growth.
  • 細胞内局在
    Cytoplasmic and Nuclear
  • 参照データベース
    • 別名
      • L0948 antibody
      • Heat shock protein 104 antibody
      • Hsp 104 antibody

    参考文献

    This product has been referenced in:
    • Hamdan N  et al. ER stress causes widespread protein aggregation and prion formation. J Cell Biol 216:2295-2304 (2017). WB ; Saccharomyces cerevisiae . Read more (PubMed: 28630146) »
    • Park YN  et al. Hsp104 overexpression cures Saccharomyces cerevisiae [PSI+] by causing dissolution of the prion seeds. Eukaryot Cell 13:635-47 (2014). WB . Read more (PubMed: 24632242) »

    See all 6 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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