HsMyoD1 ChIP probe (ab83599)
製品の概要
-
製品名
HsMyoD1 ChIP probe
MyoD1 dna・rna・cdna 製品一覧 -
特記事項
Accession number: NM_002478.The HsMYOD1 primers and probe can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells. MYOD1 (Myoblast determination protein 1) is involved in muscle development. The primers and probe are located in exon 1. In most cell types MYOD1 is enriched with histone modifications associated with silencing (e.g. Histone H3 K27 di methylation, see image with ab24684). Whereas it shows low levels of histone modifications associated with active gene transcription such as Histone H3 K9 acetylation (see image with ab4441).
-
アプリケーション
適用あり: Real Time PCRmore details
製品の特性
-
製品の状態
Liquid -
保存方法
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: Water -
Concentration information loading...
-
研究分野
-
機能
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins. -
配列類似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻訳後修飾
Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation. -
細胞内局在
Nucleus. - Information by UniProt
-
別名
- bHLHc1
- Class C basic helix-loop-helix protein 1
- MYF 3
see all
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab83599の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Real Time PCR |
Use at an assay dependent concentration.
Suitable for the analysis of chromatin immunoprecipitated DNA using Taqman® real time PCR. The probe is labelled with FAM/TAMRA. Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product. |
特記事項 |
---|
Real Time PCR
Use at an assay dependent concentration. Suitable for the analysis of chromatin immunoprecipitated DNA using Taqman® real time PCR. The probe is labelled with FAM/TAMRA. Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product. |
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab83599 は論文での使用が確認できていません。