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Accession number: NG 011433.
The HsACT1 primers can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells. Gamma actin is a constitutively expressed gene. The primers are located around the core promoter of the gamma actin gene. In most cell types the gamma actin promoter is enriched with histone modifications and core promoter binding factors (e.g. TBP, see image with ab51841) associated with active gene transcription. Together with the HsACT2 and HsACT3 primers these primers can be used to determine the abundance of a modification (e.g. Histone H3 K4 mono methylation, see image with ab8895) or a factor (e.g. RNA polymerase II, see image with ab26721) over the gamma actin locus.
Our Abpromise guarantee covers the use of ab83623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Real Time PCR||Use at an assay dependent dilution. Suitable for the analysis of chromatin immunoprecipitated DNA using Sybr® green real time PCR. Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product.|
ab83623 has not yet been referenced specifically in any publications.
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