Anti-HSF1 (phospho S303 + S307) 抗体 [EP1711Y] (ab81281)

製品の概要

  • 製品名
    Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y]
    HSF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1711Y] to HSF1 (phospho S303 + S307)
  • 特異性
    ab81281 antibody recognises HSF1 (phospho S303 + S307)
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • ポジティブ・コントロール
    • HeLa cell lysates, Human breast carcinoma.
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/50000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • 追加情報
    Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能
      DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
    • 配列類似性
      Belongs to the HSF family.
    • ドメイン
      the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
    • 翻訳後修飾
      Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
      Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
    • 細胞内局在
      Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Heat shock factor 1 antibody
      • Heat shock factor protein 1 antibody
      • Heat shock transcription factor 1 antibody
      • HSF 1 antibody
      • hsf1 antibody
      • HSF1_HUMAN antibody
      • HSTF 1 antibody
      • HSTF1 antibody
      see all

    画像

    • All lanes : Anti-HSF1 (phospho S303 + S307) antibody [EP1711Y] (ab81281) at 1/50000 dilution

      Lane 1 : HeLa cell lysate untreated
      Lane 2 : HeLa cell lysate AP treated

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRp labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 57 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma using 1/100 ab81281

    参考文献

    This product has been referenced in:
    • Asano Y  et al. IER5 generates a novel hypo-phosphorylated active form of HSF1 and contributes to tumorigenesis. Sci Rep 6:19174 (2016). WB . Read more (PubMed: 26754925) »
    • Zhao J  et al. Correlation between the expression of Id-1 and hyperthermia-associated molecules in oral squamous cell carcinoma. J Clin Pathol 66:758-63 (2013). IHC ; Human . Read more (PubMed: 23723304) »

    See all 2 Publications for this product

    レビューと Q&A

    Thank you for contacting us and bringing this to our attention.


    We have tested both products (ab76076 and ab81281) in mouse and rat samples, via WB.
    The samples we tested were brain, heart, kidney and spleen for each, in mouse and r...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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