製品の概要

  • 製品名
    Anti-HSF1 (phospho S303) antibody
    HSF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HSF1 (phospho S303)
  • アプリケーション
    適用あり: WB, IHC-P, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthesized phosphopeptide derived from HSF1 around the phosphorylation site of serine 303 (Human).

  • ポジティブ・コントロール
    • WB: Extracts from MCF cells. IHC-P: Human breast carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab47369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 57 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.
ICC/IF Use a concentration of 1 - 5 µg/ml.

ターゲット情報

  • 機能
    DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
  • 配列類似性
    Belongs to the HSF family.
  • ドメイン
    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • 翻訳後修飾
    Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
    Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
  • 細胞内局在
    Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Heat shock factor 1 antibody
    • Heat shock factor protein 1 antibody
    • Heat shock transcription factor 1 antibody
    • HSF 1 antibody
    • hsf1 antibody
    • HSF1_HUMAN antibody
    • HSTF 1 antibody
    • HSTF1 antibody
    see all

画像

  • Lanes 1 - 2 : HSF1 antibody at 1/500 dilution
    Lanes 3 - 4 : Anti-HSF1 (phospho S303) antibody (ab47369) at 1/500 dilution

    Lane 1 : Extracts from MCF cells treated with TNF alpha (20ng/ml, 30min). No peptide.
    Lane 2 : Extracts from MCF cells treated with TNF alpha (20ng/ml, 30min). Synthetic phosphopeptide present.
    Lane 3 : Extracts from MCF cells not treated with TNF alpha. No peptide.
    Lane 4 : Extracts from MCF cells treated with TNF alpha (20ng/ml, 30min). No peptide.


    Predicted band size : 57 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
  • ab47369 (1/50) staining HSF1 in paraffin-embedded human breast carcinoma tissue. Right-hand panel corresponds to a negative control for which ab47369 was pre-incubated with the immunising (blocking) peptide.
  • ICC/IF image of ab47369 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47369, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Gomez-Pastor R  et al. Abnormal degradation of the neuronal stress-protective transcription factor HSF1 in Huntington's disease. Nat Commun 8:14405 (2017). WB, IHC . Read more (PubMed: 28194040) »
  • Filone CM  et al. The master regulator of the cellular stress response (HSF1) is critical for orthopoxvirus infection. PLoS Pathog 10:e1003904 (2014). WB ; Human . Read more (PubMed: 24516381) »

See all 4 Publications for this product

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