製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to HSF1
  • 特異性
    Recognises non-sumoylated HSF1
  • アプリケーション
    適用あり: ELISA, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    PLVRVKEEPPSPPQSPR

    conjugated to KLH, corresponding to amino acids 293-309 from a central region of Human HSF1

  • ポジティブ・コントロール
    • 293 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
ICC/IF 1/10 - 1/50.

ターゲット情報

  • 機能
    DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
  • 配列類似性
    Belongs to the HSF family.
  • ドメイン
    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • 翻訳後修飾
    Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
    Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
  • 細胞内局在
    Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Heat shock factor 1 antibody
    • Heat shock factor protein 1 antibody
    • Heat shock transcription factor 1 antibody
    • HSF 1 antibody
    • hsf1 antibody
    • HSF1_HUMAN antibody
    • HSTF 1 antibody
    • HSTF1 antibody
    see all

画像

  • All lanes : Anti-HSF1 antibody (ab59963) at 1/1000 dilution

    Lane 1 : Jurkat cell line
    Lane 2 : Mouse heart tissue lysates

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 57 kDa
  • Immunofluorescent analysis staining HSF1 in HeLa using ab59963 at a 1:10 dilution. An Alexa Fluor® 488- conjugated goat anti-rabbit IgG (green) was used as the secondary antibody. DAPI was used as a nuclear stain (blue).

プロトコール

参考文献

ab59963 has not yet been referenced specifically in any publications.

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