製品の概要

  • 製品名Anti-HSF1 antibody
    HSF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HSF1
  • 特異性ab47484 detects endogenous levels of total HSF1 protein.
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    The antiserum was produced against synthesized non-phosphopeptide derived from human HSF1 around the phosphorylation site of serine 303 (P-P-SP-P-P).

  • ポジティブ・コントロール
    • Breast carcinoma tissue. MCF cells extract.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab47484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 57 kDa.
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

ターゲット情報

  • 機能DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
  • 配列類似性Belongs to the HSF family.
  • ドメインthe 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • 翻訳後修飾Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
    Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.
  • 細胞内局在Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Heat shock factor 1 antibody
    • Heat shock factor protein 1 antibody
    • Heat shock transcription factor 1 antibody
    • HSF 1 antibody
    • hsf1 antibody
    • HSF1_HUMAN antibody
    • HSTF 1 antibody
    • HSTF1 antibody
    see all

Anti-HSF1 antibody 画像

  • Staining of HSF1 on paraffin-embedded human breast carcinoma tissue using ab47484 diluted 1/50. Right panel: the anibody was incubated with the corresponding blocking peptide.
  • All lanes : Anti-HSF1 antibody (ab47484) at 1/500 dilution

    Lane 1 : Extracts from MCF cells treated with TNF-alpha (20ng/ml, 30min)
    Lane 2 : Extracts from MCF cells treated with TNF-alpha (20ng/ml, 30min) with blocking peptide


    Predicted band size : 57 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

Anti-HSF1 antibody (ab47484) 使用論文

This product has been referenced in:
  • Bellingham M  et al. Exposure to chemical cocktails before or after conception - the effect of timing on ovarian development. Mol Cell Endocrinol 376:156-72 (2013). IHC . Read more (PubMed: 23791816) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab47484.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"