1/400. Use with paraformaldehyde or methanol-fixed cells.
Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
ChIP on chip - hSET1 antibody - ChIP Grade (ab70378)
Ab70378 (10 µg) was used to immunoprecipitate chromatin from K562 cells. Immunoprecipitated and reference DNA were amplified by ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray and analyzed. As a control a similar experiment was performed using normal rabbit IgG.
Lane 1 : 293T whole cell lysate at 100 µg Lane 2 : 293T whole cell lysate at 200 µg Lane 3 : 293T nuclear extract at 100 µg Lane 4 : 293T nuclear extract at 200 µg Lane 5 : 293T whole cell lysate at 100 µg Lane 6 : 293T whole cell lysate at 200 µg Lane 7 : 293T nuclear extract at 100 µg Lane 8 : 293T nuclear extract at 200 µg
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for hSET1 using ab70378 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB.
Immunocytochemistry/ Immunofluorescence - Anti-hSET1 antibody - ChIP Grade (ab70378)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
ab70378 (1/400) staining hSET1 in assynchronous HeLa cells (green). Cells were paraformaldehyde-fixed, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.
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