Use at an assay dependent concentration. PubMed: 22067449
Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overalpping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
Lane 1 : 293T whole cell lysate at 100 µg Lane 2 : 293T whole cell lysate at 200 µg Lane 3 : 293T nuclear lysate at 100 µg Lane 4 : 293T nuclear lysate at 200 µg Lane 5 : 293T whole cell lysate at 100 µg Lane 6 : 293T whole cell lysate at 200 µg Lane 7 : 293T nuclear lysate at 100 µg Lane 8 : 293T nuclear lysate at 200 µg
Detection of Human hSET1 by Immunoprecipitation. Control IgG used in lane 1, ab70379 used at 5 µg/5mg nuclear extract from 293T cells in lane 2. Immunoprecipitated hSET1 was detected with post IP WB using ab70379 at 0.2 µg/ml. Detection: Chemiluminescence.