製品の概要

  • 製品名
    Anti-HSDL2 antibody [2A11BG6AH6]
    HSDL2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2A11BG6AH6] to HSDL2
  • アプリケーション
    適用あり: ICC, IP, In-Cell ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human liver mitochondria.

  • ポジティブ・コントロール
    • Hela cell and human liver mitochondria.
  • 特記事項

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • バッファー
    Preservative: 0.02% Sodium azide
    Constituent: HBS
  • Concentration information loading...
  • 精製度
    Ammonium Sulphate Precipitation
  • 特記事項(精製)
    Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation
  • ポリ/モノ
    モノクローナル
  • クローン名
    2A11BG6AH6
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab110298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use a concentration of 10 µg/ml.
IP Use at an assay dependent dilution.
In-Cell ELISA Use a concentration of 0.4 µg/ml. (0.4 µg/well)

ターゲット情報

  • 機能
    Has apparently no steroid dehydrogenase activity.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    Contains 1 SCP2 domain.
  • 細胞内局在
    Peroxisome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C9orf99 antibody
    • FLJ25855 antibody
    • hsdl2 antibody
    • HSDL2_HUMAN antibody
    • Hydroxysteroid dehydrogenase like protein 2 antibody
    • Hydroxysteroid dehydrogenase-like protein 2 antibody
    • MGC10940 antibody
    • MS728 antibody
    • SDR13C1 antibody
    • Short chain dehydrogenase/reductase family 13C, member 1 antibody
    see all

画像

  • As an IP antibody, HSDL2 antibody [2A11BG6AH6 ] (ab110298) works with human sample only.
  • Immunocytochemistry image of HSDL2 stained human Hela cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with HSDL2 antibody [2A11BG6AH6 ](ab110298) at 10 µg/ml for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps.

参考文献

ab110298 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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