Mouse monoclonal [2A11BG6AH6] to HSDL2
Human liver mitochondria.
Hela cell and human liver mitochondria.
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
Shipped at 4°C. Store at +4°C. Do Not Freeze.
Preservative: 0.02% Sodium azide
Concentration information loading...
Ammonium Sulphate Precipitation
Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml.
Use at an assay dependent dilution.
Use a concentration of 0.4 µg/ml. (0.4 µg/well)
Has apparently no steroid dehydrogenase activity.
Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Contains 1 SCP2 domain.
Information by UniProt
Immunoprecipitation - Anti-HSDL2 antibody [2A11BG6AH6 ] (ab110298)
As an IP antibody, HSDL2 antibody [2A11BG6AH6 ] (ab110298) works with human sample only.
Immunocytochemistry - Anti-HSDL2 antibody [2A11BG6AH6 ] (ab110298)
Immunocytochemistry image of HSDL2 stained human Hela cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with HSDL2 antibody [2A11BG6AH6 ](ab110298) at 10 µg/ml for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"