Anti-HSD3B2 抗体 [373CT9.1.3] (ab75710)
Key features and details
- Mouse monoclonal [373CT9.1.3] to HSD3B2
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-HSD3B2 antibody [373CT9.1.3]
HSD3B2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [373CT9.1.3] to HSD3B2 -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cyt (Intra)more details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Sheep, Goat, Horse, Cow, Cat, Dog, Pig, Cynomolgus monkey, Macaque monkey, Rhesus monkey -
免疫原
Synthetic peptide corresponding to Human HSD3B2 aa 1-100 conjugated to keyhole limpet haemocyanin.
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ポジティブ・コントロール
- This antibody gave a positive signal in human adrenal tissue lysate.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
373CT9.1.3 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75710の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 42 kDa). Abcam recommends blocking using 3% milk.
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Flow Cyt (Intra) |
Use 0.1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 42 kDa). Abcam recommends blocking using 3% milk. |
Flow Cyt (Intra)
Use 0.1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. -
組織特異性
Expressed in adrenal gland, testis and ovary. -
パスウェイ
Lipid metabolism; steroid biosynthesis. -
関連疾患
Defects in HSD3B2 are the cause of adrenal hyperplasia type 2 (AH2) [MIM:201810]. AH2 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: 'salt wasting' (SW, the most severe type), 'simple virilizing' (SV, less severely affected patients), with normal aldosterone biosynthesis, 'non-classic form' or late onset (NC or LOAH), and 'cryptic' (asymptomatic). In AH2, virilization is much less marked or does not occur. AH2 is frequently lethal in early life.
Note=Mild HSD3B2 deficiency in hyperandrogenic females is associated with characteristic traits of polycystic ovary syndrome, such as insulin resistance and luteinizing hormon hypersecretion. -
配列類似性
Belongs to the 3-beta-HSD family. -
細胞内局在
Endoplasmic reticulum membrane. Mitochondrion membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3283 Human
- Entrez Gene: 3284 Human
- Entrez Gene: 15492 Mouse
- Entrez Gene: 15493 Mouse
- Entrez Gene: 15494 Mouse
- Entrez Gene: 29632 Rat
- Entrez Gene: 360348 Rat
- Entrez Gene: 682974 Rat
see all -
別名
- 3 beta HSD adrenal and gonadal type antibody
- 3 beta HSD II antibody
- 3 beta HSD type II antibody
see all
画像
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Anti-HSD3B2 antibody [373CT9.1.3] (ab75710) at 1 µg/ml + Human adrenal normal tissue lysate - total protein (ab29249) at 20 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Blocked using 3% milk. -
Overlay histogram showing JEG3 cells stained with ab75710 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75710, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab75710 は 11 報の論文で使用されています。
- Wang J et al. H2 S catalysed by CBS regulates testosterone synthesis through affecting the sulfhydrylation of PDE. J Cell Mol Med 25:3460-3468 (2021). PubMed: 33713531
- Kurowska P et al. The role of vaspin in porcine corpus luteum. J Endocrinol 247:283-294 (2020). PubMed: 33108345
- Li M et al. Quercetin alleviated H2 O2 -induced apoptosis and steroidogenic impairment in goat luteinized granulosa cells. J Biochem Mol Toxicol N/A:e22527 (2020). PubMed: 32410385
- Kurowska P et al. Role of vaspin in porcine ovary: effect on signaling pathways and steroid synthesis via GRP78 receptor and protein kinase A†. Biol Reprod 102:1290-1305 (2020). PubMed: 32149334
- Maoduo Z et al. Effects of LPS on the accumulation of lipid droplets, proliferation, and steroidogenesis in goat luteinized granulosa cells. J Biochem Mol Toxicol N/A:e22329 (2019). PubMed: 30934154