製品の概要

  • 製品名Anti-HSD17B1 antibody [EP1682Y]
    HSD17B1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1682Y] to HSD17B1
  • アプリケーション適用あり: WB, ICC/IF, Flow Cyt, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HSD17B1 (C terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • WB: Human placenta lysate. IHC-P: Human placenta tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab51045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
ICC/IF 1/100 - 1/250.
Flow Cyt Use 1µg for 106 cells.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/100.
IHC-P 1/100 - 1/250.

ターゲット情報

  • 機能Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH.
  • パスウェイSteroid biosynthesis; estrogen biosynthesis.
  • 配列類似性Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 17 beta HSD 1 antibody
    • 17 beta hydroxysteroid dehydrogenase type 1 antibody
    • 17-beta-HSD 1 antibody
    • 17-beta-hydroxysteroid dehydrogenase type 1 antibody
    • 20 alpha-hydroxysteroid dehydrogenase antibody
    • 20-alpha-HSD antibody
    • DHB1_HUMAN antibody
    • E17KSR antibody
    • E2DH antibody
    • EDH17B1 antibody
    • EDH17B2 antibody
    • EDHB17 antibody
    • Estradiol 17 beta dehydrogenase 1 antibody
    • Estradiol 17-beta-dehydrogenase 1 antibody
    • HSD17 antibody
    • HSD17B1 antibody
    • Hydroxysteroid (17 beta) dehydrogenase 1 antibody
    • MGC13814 antibody
    • Placental 17 beta hydroxysteroid dehydrogenase antibody
    • Placental 17-beta-hydroxysteroid dehydrogenase antibody
    • SDR28C1 antibody
    • Short chain dehydrogenase/reductase family 28CE,member 1 antibody
    see all

Anti-HSD17B1 antibody [EP1682Y] 画像

  • Anti-HSD17B1 antibody [EP1682Y] (ab51045) at 1/100000 dilution + Human placenta lysate at 10 µg

    Secondary
    Goat anti-Rabbit HRP labeled. at 1/2000 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
  • Ab51045 (1/100 dilution) staining human HSD17B1 in paraffin embedded human placenta tissue by Immunohistochemistry.
  • Overlay histogram showing JEG3 cells stained with ab51045 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51045, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-HSD17B1 antibody [EP1682Y] (ab51045) 使用論文

This product has been referenced in:
  • Rižner TL Estrogen biosynthesis, phase I and phase II metabolism, and action in endometrial cancer. Mol Cell Endocrinol 381:124-39 (2013). Human . Read more (PubMed: 23911898) »
  • Zhang CY  et al. The contribution of 17beta-hydroxysteroid dehydrogenase type 1 to the estradiol-estrone ratio in estrogen-sensitive breast cancer cells. PLoS One 7:e29835 (2012). WB ; Human . Read more (PubMed: 22253796) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"