製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to HSD11B1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human HSD11B1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human liver tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).

ターゲット情報

  • 機能
    Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol.
  • 組織特異性
    Widely expressed. Highest expression in liver.
  • 関連疾患
    Defects in HSD11B1 are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
  • 配列類似性
    Belongs to the short-chain dehydrogenases/reductases (SDR) family.
  • 翻訳後修飾
    Glycosylated.
  • 細胞内局在
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 11 beta HSD 1 antibody
    • 11 beta HSD1 antibody
    • 11 beta hydroxysteroid dehydrogenase 1 antibody
    • 11 DH antibody
    • 11-beta hydroxysteroid dehydrogenase, type 1 antibody
    • 11-beta-HSD1 antibody
    • 11-beta-hydroxysteroid dehydrogenase 1 antibody
    • 11-DH antibody
    • 11DH antibody
    • Corticosteroid 11 beta dehydrogenase isozyme 1 antibody
    • Corticosteroid 11-beta-dehydrogenase isozyme 1 antibody
    • CORTRD2 antibody
    • DHI1_HUMAN antibody
    • HDL antibody
    • HSD 11 antibody
    • HSD11 antibody
    • HSD11B antibody
    • HSD11B1 antibody
    • HSD11L antibody
    • Hydroxysteroid (11 beta) dehydrogenase antibody
    • Hydroxysteroid (11 beta) dehydrogenase 1 antibody
    • MGC13539 antibody
    • SDR26C1 antibody
    • short chain dehydrogenase/reductase family 26C, member 1 antibody
    see all

画像

  • Anti-HSD11B1 antibody (ab84273) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 47 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

プロトコール

参考文献

ab84273 has not yet been referenced specifically in any publications.

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