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Our Abpromise guarantee covers the use of ab39364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).Can be blocked with HSD11B1 peptide (ab101097).|
|IHC-P||Use a concentration of 2 µg/ml.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20385225|
Western blot analysis of Sheep liver tissue lysate (5µg/lane) labeling HSD11B1 with ab39364 at 1/500 (16 hours at 4ºC). An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.