The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
The budding yeast Saccharomyces cerevisiae has two genes, HSP82 and HSC82, that encode homologues of HSP90. HSC82 is a molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved in cell cycle control. Cells overexpressing Hsc82 are hypersensitive to various stresses including high concentrations of NaCl. The phenotypes associated with the sensitivity to salt stress of Hsc82-overexpressing cells are similar to those of calcineurin-defective mutants.