Anti-HSC70 Interacting Protein HIP 抗体 [EP3264] (ab76005)
Key features and details
- Rabbit monoclonal [EP3264] to HSC70 Interacting Protein HIP
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-HSC70 Interacting Protein HIP antibody [EP3264]
HSC70 Interacting Protein HIP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP3264] to HSC70 Interacting Protein HIP -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: 293T and SW480 cell lysates IHC-P: Human colonic adenocarcinoma and normal colon tissues.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP3264 -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76005の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2500 - 1/5000. Predicted molecular weight: 41 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
1/2500 - 1/5000. Predicted molecular weight: 41 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
One HIP oligomer binds the ATPase domains of at least two HSC70 molecules dependent on activation of the HSC70 ATPase by HSP40. Stabilizes the ADP state of HSC70 that has a high affinity for substrate protein. Through its own chaperone activity, it may contribute to the interaction of HSC70 with various target proteins. -
配列類似性
Belongs to the FAM10 family.
Contains 1 STI1 domain.
Contains 3 TPR repeats. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 6767 Human
- Entrez Gene: 70356 Mouse
- Omim: 606796 Human
- SwissProt: P50502 Human
- SwissProt: Q99L47 Mouse
- Unigene: 712713 Human
- Unigene: 180337 Mouse
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別名
- AAG 2 antibody
- AAG2 antibody
- Aging associated protein 2 antibody
see all
画像
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All lanes : Anti-HSC70 Interacting Protein HIP antibody [EP3264] (ab76005) at 1/100000 dilution
Lane 1 : 293T cell lysate
Lane 2 : SW480 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted? -
ab76005 at 1/100 dilution staining HSC70 Interacting Protein HIP in human colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab76005 at 1/100 dilution staining HSC70 Interacting Protein HIP in human normal colon by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab76005 は 2 報の論文で使用されています。
- Dosh RH et al. Use of l-pNIPAM hydrogel as a 3D-scaffold for intestinal crypts and stem cell tissue engineering. Biomater Sci 7:4310-4324 (2019). PubMed: 31410428
- Tang S et al. Toll-like receptor 4 shRNA attenuates lipopolysaccharide-induced epithelial-mesenchymal transition of intrahepatic biliary epithelial cells in rats. Biomed Pharmacother 107:1210-1217 (2018). PubMed: 30257335