製品の概要
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製品名Anti-Hsc70 antibody
Hsc70 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to Hsc70
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由来種Goat
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特異性This antibody is expected to recognise isoform 1 (NP_006588.1) only.
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アプリケーション適用あり: WB, ELISAmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Hamster, Cow, Dog, Orangutan
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免疫原
Synthetic peptide:
C-EKLQGKINDEDKQK
, corresponding to internal sequence amino acids 556-569 of Human Hsc70 -
ポジティブ･コントロール
- A431 cell lysate
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab52057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | ||
| ELISA |
追加情報
Peptide ELISA: Antibody detection limit dilution 1:4,000.
WB: Use at a concentration of 1.0 - 3.0 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 71 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1.0 - 3.0 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 71 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
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組織特異性Ubiquitous.
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配列類似性Belongs to the heat shock protein 70 family.
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ドメインThe N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
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翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated. -
細胞内局在Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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参照データベース
- Entrez Gene: 281831 Cow
- Entrez Gene: 3312 Human
- Entrez Gene: 15481 Mouse
- Entrez Gene: 24468 Rat
- Omim: 600816 Human
- SwissProt: P11142 Human
- SwissProt: P63017 Mouse
- SwissProt: P63018 Rat
see all -
別名
- 2410008N15Rik antibody
- Constitutive heat shock protein 70 antibody
- Epididymis luminal protein 33 antibody
see all
画像
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Western blot - Anti-Hsc70 antibody (ab52057)Anti-Hsc70 antibody (ab52057) at 2 µg/ml + A431 cell lysate at 35 µg
Predicted band size: 71 kDa
Observed band size: 75 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
プロトコール
参考文献
ab52057 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"