This antibody gave a positive signal in Recombinant Hsc70 protein as well as Human Breast and Human Kidney tissue lysates and the following whole cell lysates: MCF7; MDA MB 231; HEK293.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーpH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 71 kDa).
機能Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
配列類似性Belongs to the heat shock protein 70 family.
ドメインThe N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR. ISGylated.
細胞内局在Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
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Anti-Hsc70 antibody 画像
Western blot - Anti-Hsc70 antibody (ab112549)
All lanes : Anti-Hsc70 antibody (ab112549) at 1 µg/ml
Lane 1 : Recombinant Protein:Hsc70 (Active) Lane 2 : Human breast tissue lysate - total protein (ab30090) Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 4 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 5 : Human kidney tissue lysate - total protein (ab30203) Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution developed using the ECL technique