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Full length native protein (purified) corresponding to Hamster Hsc70.
This product was changed from ascites to tissue culture supernatant on 15th August 2017. The highest lot of ascites still in stock on 15th August 2017 is GR3178418-3. Lot numbers higher than GR3178418-3 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Our Abpromise guarantee covers the use of ab19136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 73 kDa.|
|IP||Use a concentration of 5 µg/ml.|
This image is courtesy of an anonymous Abreview
This image is courtesy of an anonymous abreview.
Blocking: 5% milk for 1 hour at 22°C.
ab19136 at 1/150 dilution staining human HeLa cells by ICC/IF. The cells were heat shocked (2 hours at 42.5°C) and paraformaldehyde fixed prior to incubation with the antibody for 1 hour at 37°C. An Alexa-Fluor ® 568 conjugated goat anti-rat antibody was used as the secondary. The cells were also stained with DAPI to visualize the nuclei.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"