製品の概要

  • 製品名Anti-Hsc70 antibody [1B5]
    Hsc70 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [1B5] to Hsc70
  • アプリケーション適用あり: ICC/IF, IHC-P, ELISA, Flow Cyt, WB, IPmore details
    適用なし: IHC-Fr
  • 種交差性
    交差種: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Monkey
  • 免疫原

    Full length native protein (purified) corresponding to Hamster Hsc70.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 15th August 2017. The highest lot of ascites still in stock on 15th August 2017 is GR3178418-3. Lot numbers higher than GR3178418-3 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファーConstituent: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名1B5
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab19136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/150.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 73 kDa.
IP Use a concentration of 5 µg/ml.
  • 追加情報Is unsuitable for IHC-Fr.
  • ターゲット情報

    • 機能Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.
    • 組織特異性Ubiquitous.
    • 配列類似性Belongs to the heat shock protein 70 family.
    • ドメインThe N-terminal 1-386 residues constitute the ATPase domain, while residues 387-646 form the peptide-binding domain.
    • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
      ISGylated.
    • 細胞内局在Cytoplasm. Melanosome. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 2410008N15Rik antibody
      • Constitutive heat shock protein 70 antibody
      • Epididymis luminal protein 33 antibody
      • Epididymis secretory sperm binding protein Li 72p antibody
      • Heat shock 70 kDa protein 8 antibody
      • Heat shock 70kD protein 10 antibody
      • Heat shock 70kD protein 8 antibody
      • Heat shock 70kDa protein 8 antibody
      • Heat shock cognate 71 kDa protein antibody
      • Heat shock cognate protein 54 antibody
      • Heat shock cognate protein 71 kDa antibody
      • Heat shock protein 8 antibody
      • Heat shock protein A8 antibody
      • Heat shock protein family A (Hsp70) member 8 antibody
      • Heat-shock70-KD protein 10, formerly antibody
      • HEL 33 antibody
      • HEL S 72p antibody
      • HSC54 antibody
      • HSC71 antibody
      • Hsc73 antibody
      • HSP71 antibody
      • HSP73 antibody
      • HSP7C_HUMAN antibody
      • HSPA10 antibody
      • HSPA8 antibody
      • LAP1 antibody
      • Lipopolysaccharide associated protein 1 antibody
      • LPS associated protein 1 antibody
      • LPS associated protein antibody
      • MGC102007 antibody
      • MGC106514 antibody
      • MGC114311 antibody
      • MGC118485 antibody
      • MGC131511 antibody
      • MGC29929 antibody
      • N-myristoyltransferase inhibitor protein 71 antibody
      • NIP71 antibody
      see all

    Anti-Hsc70 antibody [1B5] 画像

    • All lanes : Anti-Hsc70 antibody [1B5] (ab19136) at 0.1 µg/ml

      Lane 1 : G361 human melanoma cells - whole cell lysate (50000 cells/lane)
      Lane 2 : T47D human breast cancer cells - whole cell lysate (50000 cells/lane)

      Secondary
      HRP conjugated rabbit anti-rat IgG
      Developed using the ECL technique

      Performed under non-reducing conditions.

      Predicted band size : 73 kDa
      Observed band size : 73 kDa


      Exposure time : 15 seconds

      This image is courtesy of an anonymous Abreview

      See Abreview

    • ab19136 staining Hsc70 in Human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/150) for 12 hours at 4°C. A Biotin-conjugated Goat anti-rat polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    • All lanes : Anti-Hsc70 antibody [1B5] (ab19136) at 1/20000 dilution

      Lane 1 : HeLa whole cell lysate
      Lane 2 : HEC59 whole cell lysate
      Lane 3 : HCT116 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      Goat anti-ray HRP conjugate at 1/10000 dilution
      Developed using the ECL technique

      Predicted band size : 73 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)


      Exposure time : 2 seconds

      This image is courtesy of an anonymous abreview.

      Blocking: 5% milk for 1 hour at 22°C.

      See Abreview

    • All lanes : Anti-Hsc70 antibody [1B5] (ab19136) at 0.1 µg/ml

      Lane 1 : Molecular weight marker
      Lane 2 : Hsc70 (Hsp73) recombinant protein
      Lane 3 : Hsp70 (Hsp72) protein
      Lane 4 : Cell lysates prepared from 3T3 cells
      Lane 5 : Cell lystes prepared from human PC-12 cells
      Lane 6 : Cell lysates prepared from CHO cells
      Lane 7 : Cell lysates prepared from GPC-16 cells
      Lane 8 : Cell lysates prepared from human Hela cells

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 73 kDa
    • ab19136 at 1/150 dilution staining human HeLa cells by ICC/IF. The cells were heat shocked (2 hours at 42.5°C) and paraformaldehyde fixed prior to incubation with the antibody for 1 hour at 37°C. An Alexa-Fluor ® 568 conjugated goat anti-rat antibody was used as the secondary. The cells were also stained with DAPI to visualize the nuclei.

      See Abreview

    • Overlay histogram showing HeLa cells stained with ab19136 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19136, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a [aRTK2758] (ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Anti-Hsc70 antibody [1B5] (ab19136) 使用論文

    This product has been referenced in:
    • Miles AL  et al. The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1a prolyl hydroxylation by regulating cellular iron levels. Elife 6:N/A (2017). WB ; Human . Read more (PubMed: 28296633) »
    • Su Hui Teo C  et al. Spatial and Temporal Resolution of Global Protein Synthesis during HSV Infection Using Bioorthogonal Precursors and Click Chemistry. PLoS Pathog 12:e1005927 (2016). Read more (PubMed: 27706239) »

    See all 18 Publications for this product

    Product Wall


    According to the protein database entry http://www.uniprot.org/uniprot/P11142
    the protein is located as follows:

    Cytoplasm. Melanosome. Nucleus › nucleolus. Note: Localized in cytoplasmic mRNP granules containing untranslated m...

    Read More

    Thank you very much for your interest in our products.

    We do not offer free or trial sizes of our products simply because we carry over 100,000 items and having multiple sizes would be logistically impossible with our automated freezers. Read More

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Hela, HEC59, HCT116)
    Loading amount 30 µg
    Specification Hela, HEC59, HCT116
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 21 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Breast)
    Specification Breast
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric buffer, pH6
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 Sep 13 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (melanoma (G361), breast cancer (T47D) cell lines)
    Loading amount 50000 cells
    Specification melanoma (G361), breast cancer (T47D) cell lines
    Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    投稿 Oct 09 2006

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Hela)
    Specification Hela
    Fixative Paraformaldehyde
    Blocking step Other as blocking agent for 30 minute(s) · Concentration: n/a
    Username

    Abcam user community

    Verified customer

    投稿 Sep 29 2006

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (cultured hippocampal neurons)
    Specification cultured hippocampal neurons
    Fixative Paraformaldehyde
    Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1%
    Username

    Dr. Vladimir Sytnyk

    Verified customer

    投稿 Aug 07 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"