製品の概要
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製品名HsAldolase ChIP probe
Aldolase dna・rna・cdna 製品一覧 -
特記事項
Accession number: NM_184043.The HsALDOA primers and probe can be can be used to quantify a control locus in chromatin immunoprecipitation (ChIP) assays with chromatin prepared from human cells. ALDOA (Fructose-bisphosphate aldolase A) is a moderately expressed housekeeping gene. The primers and probe are located in intron 2, within 1 kb of the transcription start site. In most cell types, this locus is enriched with histone modifications associated with active gene transcription such as Histone H3 K9 acetylation (see image with ab4441). Whereas it shows low levels of histone modifications associated with silencing such as Histone H3 K9 tri methylation (see image with ab8898).
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アプリケーション適用あり: Real Time PCRmore details
製品の特性
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製品の状態Liquid
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保存方法Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: None
Constituents: ddH20 -
Concentration information loading... -
研究分野
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機能Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.
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パスウェイCarbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
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関連疾患Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.
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配列類似性Belongs to the class I fructose-bisphosphate aldolase family.
- Information by UniProt
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別名
- ALDA
- Aldo1
- ALDOA
see all
アプリケーション
Our Abpromise guarantee covers the use of ab83590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
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| Real Time PCR | Use at an assay dependent concentration. Suitable for the analysis of chromatin immunoprecipitated DNA using Taqman® real time PCR. The probe is labelled with FAM/TAMRA. Quantity provided for 200 reactions. Please see the protocol page for the instructions for this product. |
参考文献
ab83590 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"