製品の概要

  • 製品名
    Anti-HS6ST3 antibody
  • 製品の詳細
    Rabbit polyclonal to HS6ST3
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide designed within residues TKQLEHQRDR QKRREERRLQ REHRDHQWPK EDGAAEGTVT EDYNSQVVRW, corresponding to C terminal amino acids 422-471 of Human HS6ST3 (NP_703157)

  • ポジティブ・コントロール
    • Fetal brain lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性
    HS6ST3 (Heparan-sulphate 6-O-sulfotransferase 1) is a single-pass type II transmembrane protein that catalyses the transfer of sulphate from PAPS (3'-phosphoadenosine 5'-phosphosulphate) to position 6 of the N-sulphoglucosamine residue of heparan sulphate. Heparan sulfate (HS) sulfotransferases, such as HS6ST3, modify HS to generate structures required for interactions between HS and a variety of proteins. These interactions are implicated in proliferation and differentiation, adhesion, migration, inflammation, blood coagulation, and other diverse processes.
  • 細胞内局在
    Membrane; Single-pass type II membrane protein
  • 参照データベース
  • 別名
    • 6OST3 antibody
    • DKFZp761K2315 antibody
    • Heparan sulfate 6-O-sulfotransferase 3 antibody
    • HS6ST 3 antibody
    • MGC144859 antibody
    • MGC144860 antibody
    see all

Anti-HS6ST3 antibody 画像

  • Anti-HS6ST3 antibody (ab82719) at 1 µg/ml + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 55 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

プロトコール

Anti-HS6ST3 antibody (ab82719) 使用論文

ab82719 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Specification
Kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TIER pH6 for 10 min. cooling down for 2 hrs in buffer
Permeabilization
No
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Jul 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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