製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to HS3ST1
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human, Chinese hamster
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 252-301 (KGFYCLRDSG RDRCLHESKG RAHPQVDPKL LNKLHEYFHE PNKKFFELVG) of Human HS3ST1 (NP_005105).

  • ポジティブ・コントロール
    • Human fetal stomach lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab91065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 24247246
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. Catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site.
  • 組織特異性
    Highly expressed in the brain and kidney and weakly expressed in the heart, lung and placenta.
  • 配列類似性
    Belongs to the sulfotransferase 1 family.
  • 細胞内局在
    Golgi apparatus lumen.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3-OST-1 antibody
    • 3OST antibody
    • 3OST1 antibody
    • EC 2.8.2.23 antibody
    • h3 OST 1 antibody
    • h3-OST-1 antibody
    • Heparan sulfate (glucosamine) 3 O sulfotransferase 1 antibody
    • Heparan sulfate 3 O sulfotransferase 1 antibody
    • Heparan sulfate 3-O-sulfotransferase 1 antibody
    • Heparan sulfate D glucosaminyl 3 O sulfotransferase 1 antibody
    • Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1 antibody
    • Heparan sulfate glucosamine 3-O-sulfotransferase 1 antibody
    • Heparin glucosamine 3 O sulfotransferase antibody
    • HS3S1_HUMAN antibody
    • HS3ST 1 antibody
    • Hs3st1 antibody
    see all

画像

  • Anti-HS3ST1 antibody (ab91065) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal stomach lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Gel concentration: 12%
  • Cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature and permeabilized with permeabilization buffer + 10% FBS in PBS for 10 minutes. Cells were incubated with ab91065 and goat anti-GM130 Golgi marker (ab1299) overnight at 4°C in the dark. Cells were washed once and stained with Alexa Fluor 647-conjugated donkey anti-rabbit IgG and Alexa Fluor 488-conjugated donkey anti-goat IgG secondary antibodies. DAPI - DNA. Dual-29 cells were used as a positive control, and the other cell samples were then evaluated at the same frequency. Overexpression of HS3st1 in Dual-29 cells resulted in a broad distribution of HS3st1. Overexpression of HS3st1 in Dual-10 cells localized HS3st1 in the Golgi. Overexpression of Golgi-targeted HS3st1 in CHO-gt31 localized HS3st1 in the Golgi.

参考文献

This product has been referenced in:
  • Datta P  et al. Bioengineered Chinese hamster ovary cells with Golgi-targeted 3-O-sulfotransferase-1 biosynthesize heparan sulfate with an antithrombin-binding site. J Biol Chem 288:37308-18 (2013). ICC/IF ; Chinese hamster . Read more (PubMed: 24247246) »

See 1 Publication for this product

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