製品の概要

  • 製品名
  • 製品の詳細
    Rat polyclonal to HRP
  • 特異性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Peroxidase [Horseradish]. Cross reactivity against Peroxidase from other tissues may occur but have not been specifically determined.
  • アプリケーション
    適用あり: Dot blot, WB, ELISA, IPmore details
  • 種交差性
    Reacts with Horseradish.
  • 免疫原

    Full length Horseradish Peroxidase.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Constituents: 0.87% Sodium chloride, 0.164% Sodium phosphate
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 特記事項(精製)
    This product was prepared from monospecific antiserum by a delipidation and defibrination.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab34827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot
WB
ELISA
IP
  • 追加情報
    Dot: Use at an assay dependent dilution.
    ELISA: 1/1000 - 1/6000. This product has been assayed against 1.0µg of Peroxidase [Horseradish] in a standard sandwich ELISA using Alklaine Phosphatase conjugated affinity purified anti-Rat IgG [H&L] (Goat) and pNPP as a substrate for 30 minutes at RT.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 35 kDa.

    Suitable for most immunological methods requiring high titer and specificity.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
    • 細胞内局在
      Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.
    • 別名
      • Horseradish Peroxidase antibody
      • HPRC1 antibody
      • HRP antibody
      • Peroxidase antibody
      • Peroxidase C1A antibody
      • PRXC1A antibody
      see all

    参考文献

    ab34827 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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