製品の概要

  • 製品名Anti-HRP antibody [2H11cc]
    HRP 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2H11cc] to HRP
  • 特異性Horseradish peroxidase isoforms with pI from 3.5 to 9.5. ab10183 does not influence enzymatic activity upon immune complex formation.
  • アプリケーション適用あり: ELISA, IHC-P, IHC-Frmore details
  • 免疫原

    Purified peroxidase from horseradish.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.
  • ポリ/モノモノクローナル
  • クローン名2H11cc
  • ミエローマSp2/0
  • アイソタイプIgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab10183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 関連性Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
  • 細胞内局在Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.
  • 別名
    • Horseradish Peroxidase antibody
    • HPRC1 antibody
    • HRP antibody
    • Peroxidase antibody
    • Peroxidase C1A antibody
    • PRXC1A antibody
    see all

Anti-HRP antibody [2H11cc] (ab10183) 使用論文

This product has been referenced in:
  • Shazeeb MS  et al. Targeted signal-amplifying enzymes enhance MRI of EGFR expression in an orthotopic model of human glioma. Cancer Res 71:2230-9 (2011). IHC-Fr . Read more (PubMed: 21245103) »
  • Terashima H  et al. A sensitive short-term evaluation of antifibrotic effects using newly established type I collagen reporter transgenic rats. Am J Physiol Renal Physiol 299:F792-801 (2010). IHC-Fr . Read more (PubMed: 20660018) »

See all 3 Publications for this product

Product Wall

Thank you for your enquiry. Unfortunately, the HRP epitope that this antibody recognizes has not been determined. However, it is likely to recognizes the protein part of the molecule because it reacts with various peroxidase strains differing in com...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"