製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Hrk
  • アプリケーション
    適用あり: ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    AQLTAARLKALGDELHQRTMW

    conjugated to KLH, corresponding to amino acids 30-50 of Human Hrk

  • ポジティブ・コントロール
    • Human breast carcinoma tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    ab59907 waa purified through a protein G column and eluted out with both high and low pH buffers. It was neutralized immediately after elution then followed by dialysis against PBS.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 関連性
    Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3) group of the Bcl-2 family of proteins, was identified as a novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL. Despite its predicted molecular weight, Hrk often migrates at 12-15 kDa.
  • 参照データベース
  • 別名
    • Activator of apoptosis harakiri antibody
    • Activator of apoptosis Hrk antibody
    • BCL2 interacting protein antibody
    • BH3 interacting domain protein 3 antibody
    • BID3 antibody
    • DP5 antibody
    • Harakiri BCL2 interacting protein antibody
    • Human brain 3UTR of mRNA for neuronal death protein partial sequence antibody
    • Neuronal death protein DP5 antibody
    see all

画像

  • ab59907, at a 1/50 dilution, staining Hrk in formalin-fixed, paraffin-embedded human breast carcinoma tissue by Immunohistochemistry. The primary antibody was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

プロトコール

参考文献

This product has been referenced in:
  • Barrera-Vilarmau S  et al. Intrinsic order and disorder in the bcl-2 member harakiri: insights into its proapoptotic activity. PLoS One 6:e21413 (2011). ELISA ; Human . Read more (PubMed: 21731739) »
  • Sborgi L  et al. Characterization of a Novel Interaction between Bcl-2 Members Diva and Harakiri. PLoS One 5:e15575 (2010). ELISA ; Human . Read more (PubMed: 21209886) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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