製品の概要

  • 製品名Anti-Hrk antibody
    Hrk 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Hrk
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide of 15 amino acids in length from near the center of human Hrk

  • ポジティブ・コントロール
    • Mouse pancreas tissue lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab45419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 10 kDa).

ターゲット情報

  • 関連性Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3) group of the Bcl-2 family of proteins, was identified as a novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL. Despite its predicted molecular weight, Hrk often migrates at 12-15 kDa.
  • 参照データベース
  • 別名
    • Activator of apoptosis harakiri antibody
    • Activator of apoptosis Hrk antibody
    • BCL2 interacting protein antibody
    • BH3 interacting domain protein 3 antibody
    • BID3 antibody
    • DP5 antibody
    • Harakiri BCL2 interacting protein antibody
    • Human brain 3UTR of mRNA for neuronal death protein partial sequence antibody
    • Neuronal death protein DP5 antibody
    see all

Anti-Hrk antibody 画像

  • Lane 1 : Anti-Hrk antibody (ab45419) at 2.5 µg/ml
    Lane 2 : Anti-Hrk antibody (ab45419) at 5 µg/ml

    Lane 1 : Mouse pancreas tissue lysate
    Lane 2 : Mouse pancreas tissue lysate


    Predicted band size : 10 kDa
    Observed band size : 13 kDa (why is the actual band size different from the predicted?)
  • ab45419 (2ug/ml) staining Hrk in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of Hrk in cytoplasm of the germinal epithelium.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 using a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab45419 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45419, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Hrk antibody (ab45419) 使用論文

ab45419 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (lymphoma cell lines)
Loading amount 50 µg
Specification lymphoma cell lines
Gel Running Conditions Reduced Denaturing (4)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Dr. linfeng chen

Verified customer

投稿 Sep 22 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"