製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to HRH4
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Dog, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Mouse HRH4.

    (Peptide available as ab109635.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Raw264.7 and EL4 whole cell lysates as well as the following tissue lysates: Mouse brain; Mouse bone marrow; Mouse spleen; Mouse thymus.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).

ターゲット情報

  • 機能
    The H4 subclass of histamine receptors could mediate the histamine signals in peripheral tissues. Displays a significant level of constitutive activity (spontaneous activity in the absence of agonist).
  • 組織特異性
    Expressed primarily in the bone marrow and eosinophils. Shows preferential distribution in cells of immunological relevance such as T-cells, dendritic cells, monocytes, mast cells, neutrophils. Also expressed in a wide variety of peripheral tissues, including the heart, kidney, liver, lung, pancreas, skeletal muscle, prostate, small intestine, spleen, testis, colon, fetal liver and lymph node.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AXOR35 antibody
    • BG26 antibody
    • G protein coupled receptor 105 antibody
    • G-protein coupled receptor 105 antibody
    • GPCR105 antibody
    • GPRv53 antibody
    • H4 antibody
    • H4R antibody
    • HH4R antibody
    • Histamine H4 receptor antibody
    • Histamine receptor H4 antibody
    • Hrh4 antibody
    • HRH4_HUMAN antibody
    • Pfi-013 antibody
    • SP9144 antibody
    see all

画像

  • All lanes : Anti-HRH4 antibody (ab97997) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Mouse Bone Marrow Tissue Lysate
    Lane 3 : Spleen (Mouse) Tissue Lysate
    Lane 4 : Thymus (Mouse) Tissue Lysate
    Lane 5 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate
    Lane 6 : EL4 whole cell lysate (ab7183)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 68 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

プロトコール

参考文献

ab97997 has not yet been referenced specifically in any publications.

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