The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to HRG beta 1 (ab87547). When used as detection antibody when paired with recommended antibody, allows detection at least 0.2 - 0.4 ng/well of recombinant human HRG beta 1(using 100 µl/well antibody solution).
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 70 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant human HRG beta 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
HRG beta 1 is an isoform from the Neuregulin/Heregulin family of structurally related polypeptide growth factors which are derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells.
HRG1-ß1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. Although HRG1-ß1 biological effects are still unclear, it has been found to promote motility and invasiveness of breast cancer cells which may also involve up-regulation of expression and function of the autocrine motility promoting factor (AMF).