製品の概要
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製品名Anti-HRG beta 1 antibody (Biotin)
HRG beta 1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to HRG beta 1 (Biotin)
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由来種Rabbit
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標識Biotin
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アプリケーション適用あり: Sandwich ELISA, WBmore details
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種交差性交差種: Human
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免疫原
Recombinant Human HRG beta 1.
製品の特性
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製品の状態Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: None
Constituents: PBS, pH 7.2 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）ab87549 was purified by affinity chromatography and then biotinylated.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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sELISA pair antibody
アプリケーション
Our Abpromise guarantee covers the use of ab87549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA | Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to HRG beta 1 (ab87547). When used as detection antibody when paired with recommended antibody, allows detection at least 0.2 - 0.4 ng/well of recombinant human HRG beta 1(using 100 µl/well antibody solution). | |
| WB | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 70 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant human HRG beta 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
ターゲット情報
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関連性HRG beta 1 is an isoform from the Neuregulin/Heregulin family of structurally related polypeptide growth factors which are derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells. HRG1-ß1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. Although HRG1-ß1 biological effects are still unclear, it has been found to promote motility and invasiveness of breast cancer cells which may also involve up-regulation of expression and function of the autocrine motility promoting factor (AMF).
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細胞内局在Secreted
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参照データベース
- Entrez Gene: 3084 Human
- Omim: 142445 Human
- SwissProt: Q02297 Human
- Unigene: 453951 Human
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別名
- Acetylcholine receptor inducing activity antibody
- ARIA antibody
- Breast cancer cell differentiation factor p45 antibody
see all
プロトコール
参考文献
ab87549 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"