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Our Abpromise guarantee covers the use of ab87547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to HRG beta 1 (Biotin) (ab87549). Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human HRG beta 1. Can be used as capture antibody when paired with ab87549.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 70 kDa.|
IHC image of HRG beta 1 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87547, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab87547 has not yet been referenced specifically in any publications.