The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 12 kDa.
Use at an assay dependent dilution.
Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.
IP analysis of HPV18 E7 protein using ab100953 at 1/500: Lane 1: detection of E7 protein in lysate of U20S cells
Lane 2: the lower band is precipitated E7, upper bands are light and heavy chain of antibody
Lane 3: precipitation without ab100953
Lane 4: IP exp. with plain U2OS cells
Lane 5: IP exp. with plain Hela cells, where the lower band is non specific binding
Lane 6: marker.
Immunofluorescent (IF) analysis of HPV18 E7 protein in U2OS cells. IF was carried out with U2OS cells transfected with plasmid expressing HPV18 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV18 E7 detected by ab100953. IF analysis of E7 protein shows similar result also in HeLa cells.
Wasson CW et al. Human papillomavirus type 18 E5 oncogene supports cell cycle progression and impairs epithelial differentiation by modulating growth factor receptor signalling during the virus life cycle. Oncotarget8:103581-103600 (2017).
Read more (PubMed: 29262586) »
Ohba K et al. In Vivo and In Vitro Studies Suggest a Possible Involvement of HPV Infection in the Early Stage of Breast Carcinogenesis via APOBEC3B Induction. PLoS One9:e97787 (2014).
Read more (PubMed: 24858917) »