製品の概要

  • 製品名
    Anti-HPV18 E2 antibody [2E7]
  • 製品の詳細
    Mouse monoclonal [2E7] to HPV18 E2
  • 特異性
    Reacts in WB, and ELISA with HPV18 native E2 protein as well recombinant E2 protein expressed in bacteria and Cos 7 cells.
  • アプリケーション
    適用あり: WB, ELISAmore details
    適用なし: IP
  • 種交差性
    交差種: Human papillomavirus
  • 免疫原

    Recombinant full length protein corresponding to HPV18 E2.

  • エピトープ
    Mapped to amino acids 1-83.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    Purified from bacteria by affinity chromatography.
  • ポリ/モノ
    モノクローナル
  • クローン名
    2E7
  • アイソタイプ
    IgG

アプリケーション

Our Abpromise guarantee covers the use of ab100969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 µg/ml. Predicted molecular weight: 41 kDa.
ELISA Use a concentration of 0.1 µg/ml.
  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 関連性
      Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.
    • 細胞内局在
      Host nucleus
    • 参照データベース
      • 別名
        • E2 antibody
        • Human papillomavirus type 18 E2 protein antibody
        • Regulatory protein E2 antibody

      Anti-HPV18 E2 antibody [2E7] 画像

      • All lanes : Anti-HPV18 E2 antibody [2E7] (ab100969) at 1/100 dilution

        Lane 1 : lysates of U20S cells transfected with plasmid expressing HPV11 E2 protein
        Lane 2 : lysates of plain U20S cells
        Lane 3 : lysates of plain HeLa cells
        Lane 4 : lysates of U20S transfected with plasmid expressing HPV11 E2 protein
        Lane 5 : marker


        Predicted band size : 41 kDa
      • Immunofluorescence (IF) analysis of HPV18 E2 protein in U2OS cells. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV18 E2 detected by ab100969.

      プロトコール

      Anti-HPV18 E2 antibody [2E7] (ab100969) 使用論文

      ab100969 has not yet been referenced specifically in any publications.

      Product Wall

      There are currently no Abreviews or Questions for ab100969.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

      登録