製品の概要

  • 製品名Anti-HPV11 E7 antibody [9H5]
    HPV11 E7 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [9H5] to HPV11 E7
  • 特異性Reacts in WB, IP, ELISA with HPV11 native E7 protein as well as recombinant E7 protein expressed in bacteria and Cos7 cells.
  • アプリケーション適用あり: WB, IP, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human Papillomavirus (HPV)
  • 免疫原

    Recombinant full length HPV11 E7 protein purified from bacteria.

  • エピトープMapped to amino acids 36-70
  • ポジティブ・コントロール
    • WB: U2OS and HeLa cell lysates ICC/IF: U2OS cells transfected with plasmid expressing HPV-11 E7 protein IP: lysate of U20S cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)Purified from bacteria by affinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名9H5
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab100967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 11 kDa.
IP 1/500.
ELISA 1/128000.
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • 関連性Infections by human papillomaviruses (HPV) can cause relatively harmless plantar warts and also more serious genital warts that can increase the risk to women for developing cervical cancer. Infections with HPV11 triggers genital warts. E7 is a protein that is encoded by by HPV11 and is implicated in the disruption of host cells and promoting their proliferation.
  • 参照データベース
    • 別名
      • Human papillomavirus E7 protein antibody

    Anti-HPV11 E7 antibody [9H5] 画像

    • All lanes : Anti-HPV11 E7 antibody [9H5] (ab100967) at 1/1000 dilution

      Lane 1 : lysate of U20S cells transfected with plasmid expressing HPV11 E7 protein
      Lane 2 : lysate of plain U20S cells
      Lane 3 : lysate of HeLa cells
      Lane 4 : lysate of U20S cells transfected with plasmid expressing HPV11 E7 protein
      Lane 5 : marker


      Predicted band size : 11 kDa
    • Immunoprecipitation (IP) analysis of HPV11 E7 protein.
      Lane 1 represents the marker.
      Lane 2 shows detection of E7 protein by ab100967 in lysate of U20S cells.
      In lane 3 the lower band is precipitated E7, upper bands are light and heavy chain of the antibody.
      Lane 4 is precipitation without ab100967
      Lane 5 shows IP experiment with plain U2OS cells.
    • Immunofluorescence (IF) analysis of HPV11 E7 protein in U2OS cells.
      IF was carried out using U2OS cells transfected with plasmid expressing HPV11 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV11 E7 detected by ab100967. IF analysis of E7 protein shows similar result also in HeLa cells.

    Anti-HPV11 E7 antibody [9H5] (ab100967) 使用論文

    ab100967 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"