The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 42 kDa.
Use at an assay dependent dilution.
Is unsuitable for IP.
Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.
Western blot - HPV11 E2 antibody [10E12] (ab100968)
All lanes : Anti-HPV11 E2 antibody [10E12] (ab100968) at 1/1000 dilution
Lane 1 : lysate of U20S cells transfected with plasmid expressing
HPV11 E2 protein Lane 2 : lysate of plain U20S cells Lane 3 : lysate of plain HeLa cells Lane 4 : lysate of U20S cells transfected with plasmid expressing
HPV11 E2 protein Lane 5 : marker
Immunofluorescence (IF) analysis of HPV11 E2 protein in U2OS cells. IF was carried out with U2OS cell transfected with plasmid expressing HPV11 E2 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV11 E2 detected by ab100968. IF analysis of E2 protein shows similar result also in HeLa cells.