製品の概要

  • 製品名Anti-HPV11 E2 antibody [10E12]
  • 製品の詳細
    Mouse monoclonal [10E12] to HPV11 E2
  • 特異性Reacts in WB, and ELISA with HPV11 native E2 protein as well as recombinant E2 protein expressed in bacteria and Cos 7 cells.
  • アプリケーション適用あり: WB, ELISA, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Human Papillomavirus (HPV)
  • 免疫原

    Recombinant full length HPV11 E2 protein purified from bacteria.

  • エピトープMapped to amino acids 202-284
  • ポジティブ・コントロール
    • U20S or Hela cells and lysates

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)Purified from bacteria by affinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名10E12
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab100968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 42 kDa.
ELISA 1/32000.
ICC/IF Use at an assay dependent dilution.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 関連性Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.
    • 細胞内局在Host nucleus
    • 参照データベース
      • 別名
        • Human papillomaviruses 11 E2 antibody

      Anti-HPV11 E2 antibody [10E12] 画像

      • All lanes : Anti-HPV11 E2 antibody [10E12] (ab100968) at 1/1000 dilution

        Lane 1 : lysate of U20S cells transfected with plasmid expressing HPV11 E2 protein
        Lane 2 : lysate of plain U20S cells
        Lane 3 : lysate of plain HeLa cells
        Lane 4 : lysate of U20S cells transfected with plasmid expressing HPV11 E2 protein
        Lane 5 : marker


        Predicted band size : 42 kDa
      • Immunofluorescence (IF) analysis of HPV11 E2 protein in U2OS cells.
        IF was carried out with U2OS cell transfected with plasmid expressing HPV11 E2 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV11 E2 detected by ab100968. IF analysis of E2 protein shows similar result also in HeLa cells.

      Anti-HPV11 E2 antibody [10E12] (ab100968) 使用論文

      ab100968 has not yet been referenced specifically in any publications.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"