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Internal synthetic peptide:
Our Abpromise guarantee covers the use of ab40827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2 - 3 µg/ml.|
|ELISA||Use at an assay dependent dilution.|
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).|
ab40827 at 2.5µg/ml staining HP1 gamma in human placenta tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"