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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HP1 alpha.
(Peptide available as ab4718.)
This antibody was thought to work in plants, but the localisation patterns, though specific, were not co-localised with methylated Histone H3 K9 as is found in other species.
Our Abpromise guarantee covers the use of ab9057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa).Can be blocked with HP1 alpha peptide (ab4718).|
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab9057 overnight at 4°C. Antibody binding was detected using an anti-rabbit IgG VHH single domain antibody (ab191866)conjugated to HRP, and visualised using ECL development solution ab133406.
Detection of ab9057 in a blood sample. This result is compatible with other results obtained with other HP1 antibodies (Minc et al., 2000 Cytogenet Cell Genet 90:279 –284). (Manuela Silva, personal communication).