Anti-HP1 alpha 抗体 (ab77256)
Key features and details
- Goat polyclonal to HP1 alpha
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-HP1 alpha antibody
HP1 alpha 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to HP1 alpha -
由来種
Goat -
アプリケーション
適用あり: ICC/IF, Flow Cyt, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Cow, Dog, Rhesus monkey, Chinese hamster -
免疫原
Synthetic peptide:
C-NKRKSNFSNSADDIK
, corresponding to internal sequence amino acids 88-102 of Human HP1 alpha (NP_036249.1). -
ポジティブ・コントロール
- WB: A43N, K562 cell lysates. ICC/IF: U-2 OS cells. Flow Cyt: HeLa cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
ab77256 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77256の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | (4) |
Use a concentration of 10 µg/ml.
|
Flow Cyt |
Use a concentration of 10 µg/ml.
|
|
WB | (5) |
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Primary incubation 1 hour at room temperature reccomended for this product. |
特記事項 |
---|
ICC/IF
Use a concentration of 10 µg/ml. |
Flow Cyt
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). Primary incubation 1 hour at room temperature reccomended for this product. |
ターゲット情報
-
機能
Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. -
配列類似性
Contains 2 chromo domains. -
翻訳後修飾
Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Ubiquitinated. -
細胞内局在
Nucleus. Chromosome. Chromosome > centromere. Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase. - Information by UniProt
-
参照データベース
- Entrez Gene: 538885 Cow
- Entrez Gene: 477593 Dog
- Entrez Gene: 23468 Human
- Entrez Gene: 300266 Rat
- Omim: 604478 Human
- SwissProt: P45973 Human
- Unigene: 349283 Human
-
別名
- Antigen p25 antibody
- CBX5 antibody
- CBX5_HUMAN antibody
see all
画像
-
All lanes : Anti-HP1 alpha antibody (ab77256) at 0.03 µg/ml
Lane 1 : A43N cell lysate
Lane 2 : K562 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 22 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted? -
Immunofluorescence analysis of paraformaldehyde fixed, 0.15% Triton permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling HP1 alpha with ab77256 at 10ug/ml for 1hr, followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
-
Flow cytometric analysis HeLa cells labeling HP1 alpha with ab77256 at 10 ug/mL (blue line). Cells were fixed with paraformaldehyde fixed and permeabilized with 0.5% Triton. Alexa Fluor 488 at 2ug/mL was used as the secondary antibody. IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (38)
ab77256 は 38 報の論文で使用されています。
- Hsia CR et al. Confined migration induces heterochromatin formation and alters chromatin accessibility. iScience 25:104978 (2022). PubMed: 36117991
- Wang Q et al. The H2A.Z-KDM1A complex promotes tumorigenesis by localizing in the nucleus to promote SFRP1 promoter methylation in cholangiocarcinoma cells. BMC Cancer 22:1166 (2022). PubMed: 36368958
- Noches V et al. Pilocarpine-induced seizures associate with modifications of LSD1/CoREST/HDAC1/2 epigenetic complex and repressive chromatin in mice hippocampus. Biochem Biophys Rep 25:100889 (2021). PubMed: 33426312
- Himeda CL et al. Targeted epigenetic repression by CRISPR/dSaCas9 suppresses pathogenic DUX4-fl expression in FSHD. Mol Ther Methods Clin Dev 20:298-311 (2021). PubMed: 33511244
- Kong NR et al. Zinc Finger Protein SALL4 Functions through an AT-Rich Motif to Regulate Gene Expression. Cell Rep 34:108574 (2021). PubMed: 33406418