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Our Abpromise guarantee covers the use of ab77256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 2 - 3 µg/ml.|
|ChIP||Use at an assay dependent dilution. PubMed: 20663865|
|WB||Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).|
Primary incubation was 1 hour. Detected by chemiluminescence.
ab77256 at 2.5µg/ml staining HP1 alpha in human uterus tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
ab77256 (2µg/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.