Anti-HOXC9 抗体 [HOXCA6E6] (ab50839)
Key features and details
- Mouse monoclonal [HOXCA6E6] to HOXC9
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-HOXC9 antibody [HOXCA6E6]
HOXC9 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [HOXCA6E6] to HOXC9 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
HOXC9 recombinant fragment (Human), containing 50-200 aa
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ポジティブ・コントロール
- Hela cells and HT-1080 whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum, filtered through a 0.22µm membrane. -
ポリ/モノ
モノクローナル -
クローン名
HOXCA6E6 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50839の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | (4) |
1/5.
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WB | (1) |
1/100. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
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特記事項 |
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ICC/IF
1/5. |
WB
1/100. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
ターゲット情報
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機能
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. -
配列類似性
Belongs to the Abd-B homeobox family.
Contains 1 homeobox DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3225 Human
- Omim: 142971 Human
- SwissProt: P31274 Human
- Unigene: 658823 Human
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別名
- Homeo box C9 antibody
- Homeobox C9 antibody
- Homeobox protein Hox C9 antibody
see all
画像
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Anti-HOXC9 antibody [HOXCA6E6] (ab50839) at 1/100 dilution + HT-1080 whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa -
ab50839 at a 1/5 dilution staining HOXC9 in Hela cells using Alexa Fluor® 488 Goat Anti-mouse IgG at a 1/200 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab50839 は 9 報の論文で使用されています。
- Wind M & Tsakiridis A In Vitro Generation of Posterior Motor Neurons from Human Pluripotent Stem Cells. Curr Protoc 1:e244 (2021). PubMed: 34547185
- Faustino Martins JM et al. Self-Organizing 3D Human Trunk Neuromuscular Organoids. Cell Stem Cell 26:172-186.e6 (2020). PubMed: 31956040
- Frith TJR & Tsakiridis A Efficient Generation of Trunk Neural Crest and Sympathetic Neurons from Human Pluripotent Stem Cells Via a Neuromesodermal Axial Progenitor Intermediate. Curr Protoc Stem Cell Biol N/A:e81 (2019). PubMed: 30688409
- Dobrotkova V et al. Prediction of neuroblastoma cell response to treatment with natural or synthetic retinoids using selected protein biomarkers. PLoS One 14:e0218269 (2019). PubMed: 31188873
- Ichida JK et al. Comparative genomic analysis of embryonic, lineage-converted and stem cell-derived motor neurons. Development 145:N/A (2018). PubMed: 30337375
- Frith TJ et al. Human axial progenitors generate trunk neural crest cells in vitro. Elife 7:N/A (2018). PubMed: 30095409
- Stoll SJ et al. HOXC9 regulates formation of parachordal lymphangioplasts and the thoracic duct in zebrafish via stabilin 2. PLoS One 8:e58311 (2013). PubMed: 23484014
- Stoll SJ et al. The Transcription Factor HOXC9 Regulates Endothelial Cell Quiescence and Vascular Morphogenesis in Zebrafish via Inhibition of Interleukin 8. Circ Res 108:1367-77 (2011). PubMed: 21493894
- Patani R et al. Retinoid-independent motor neurogenesis from human embryonic stem cells reveals a medial columnar ground state. Nat Commun 2:214 (2011). PubMed: 21364553