製品の概要

  • 製品名Anti-HOXC8 antibody
    HOXC8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HOXC8
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Sheep, Chicken, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HOXC8.

    (Peptide available as ab101082.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Mouse Spinal Cord and Mouse Brain Tissue Lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).

ターゲット情報

  • 機能Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • 配列類似性Belongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Homeo box 3A antibody
    • Homeo box 8 antibody
    • Homeobox 3A antibody
    • Homeobox C8 antibody
    • Homeobox protein Hox C8 antibody
    • Homeobox protein Hox-3A antibody
    • Homeobox protein Hox-C8 antibody
    • Homeobox protein HoxC8 antibody
    • Homeobox protein R4 antibody
    • Homolog of mouse Hox 3.1 antibody
    • HOX 3 antibody
    • Hox 3.1 mouse homolog of antibody
    • HOX 3A antibody
    • HOX C8 antibody
    • Hox-3.1 antibody
    • Hox-3A antibody
    • HOX3 antibody
    • HOX3A antibody
    • HOXC 8 antibody
    • Hoxc-8 antibody
    • HOXC8 antibody
    • HXC8_HUMAN antibody
    • M31 antibody
    see all

Anti-HOXC8 antibody 画像

  • All lanes : Anti-HOXC8 antibody (ab79690) at 1 µg/ml

    Lane 1 : Spinal Cord (Mouse) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa


    Exposure time : 16 minutes
  • ICC/IF image of ab79690 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79690, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-HOXC8 antibody (ab79690) 使用論文

ab79690 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"