製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Hoxb3
  • アプリケーション
    適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide within residues: GPSKSGPPKC GPGTNSTLTK QIFPWMKESR QTSKLKNNSP GTAEGCGGGG , corresponding to N terminal amino acids 108-157 of Human Hoxb3 (NP_002137)

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 25745994
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

ターゲット情報

  • 機能
    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • 配列類似性
    Belongs to the Antp homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 発生段階
    Expressed in whole embryos and fetuses at 5-9 weeks from conception.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Homeo box 2G antibody
    • Homeo box B3 antibody
    • Homeobox B3 antibody
    • Homeobox protein Hox-2.7 antibody
    • Homeobox protein Hox-2G antibody
    • Homeobox protein Hox-B3 antibody
    • Homeobox protein HoxB3 antibody
    • Hox 2.7 antibody
    • HOX 2G antibody
    • HOX2 antibody
    • Hox2.7 antibody
    • HOX2G antibody
    • HOXB3 antibody
    • HXB3_HUMAN antibody
    see all

画像

  • Anti-Hoxb3 antibody (ab83404) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa

プロトコール

参考文献

This product has been referenced in:
  • Jiang Y  et al. Repression of Hox genes by LMP1 in nasopharyngeal carcinoma and modulation of glycolytic pathway genes by HoxC8. Oncogene 34:6079-91 (2015). IHC-P ; Human . Read more (PubMed: 25745994) »

See 1 Publication for this product

レビューと Q&A

I have processed your request to receive a refund for ab83404 which did not work in western blot. Your credit note number is ******.

Thank you for your reply and for providing the order details. ab843404 is covered under our Abpromsie and so is guaranteed to work in western blotting on human samples. As it is clearly not working as promised, then I can either send you a replacement ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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