製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to HOXA9
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 223-272 (KEFLFNMYLT RDRRYEVARL LNLTERQVKI WFQNRRMKMK KINKDRAKDE) of Human HOXA9 (NP_689952).

  • ポジティブ・コントロール
    • 721_B cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
  • 関連疾患
    Note=A chromosomal aberration involving HOXA9 is found in a form of acute myeloid leukemia. Translocation t(7;11)(p15;p15) with NUP98.
    Note=A chromosomal aberration involving HOXA9 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with MSI2.
  • 配列類似性
    Belongs to the Abd-B homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 製品の状態
    HOXA9 is a transcription factor with a central role in both haemopoiesis and leukaemia. High levels of HOXA9 expression in haemopoietic cells is a characteristic feature of acute myeloid leukaemia (AML), and may be sufficient to cause this disease. Overexpression of Hoxa 9 markedly expands hematopoietic stem cells.
  • 別名
    • ABD-B antibody
    • Abd-B, drosophila, homolog of antibody
    • D6a9 antibody
    • Homeobox 1G antibody
    • Homeobox A9 antibody
    • Homeobox protein Hox-1.7 antibody
    • Homeobox protein Hox-1G antibody
    • Homeobox protein Hox-A9 antibody
    • Homeodomain protein HOXA9 antibody
    • Hox-1.7 antibody
    • Hox-1.7, mouse, homolog of antibody
    • HOX1 antibody
    • Hox1.7 antibody
    • Hox1G antibody
    • Hox1r5 antibody
    • Hoxa-9 antibody
    • Hoxa7 antibody
    • HOXA9 antibody
    • HOXA9/MSI2 fusion gene, included antibody
    • HOXA9/NUP98 fusion gene, included antibody
    • HXA9_HUMAN antibody
    • MGC1934 antibody
    see all

画像

  • Anti-HOXA9 antibody (ab83480) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 30 kDa
  • ICC/IF image of ab83480 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83480, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:

See 1 Publication for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus laevis Cell (spinal cord)
Permeabilization
Yes - 0.1% Tween
Specification
spinal cord
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
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Ms. Zahra Motahari

Verified customer

投稿 Aug 04 2015

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees ...

Read More

Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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