製品の概要

  • 製品名Anti-HOXA5 antibody
    HOXA5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HOXA5
  • アプリケーション適用あり: IHC-P, IHC-Fr, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 215-264 (FNRYLTRRRR IEIAHALCLS ERQIKIWFQN RRMKWKKDNK LKSMSMAAAG) of Human HOXA5 (NP_061975).

  • ポジティブ・コントロール
    • Placental lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 - 10 µg/ml.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 29, 65 kDa (predicted molecular weight: 29 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

Anti-HOXA5 antibody 画像

  • Anti-HOXA5 antibody (ab82645) at 1 µg/ml + placental lysate at 10 µg

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  • ab82645 at 5 µg/ml staining HOXA5 in Human breast tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab82645 at 5 µg/ml staining HOXA5 in Human kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab82645 staining HOXA5 in fetal mouse lung tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed with paraformaldehyde, permeabilized using PBST, blocked with 1.5% serum for 20 minutes at 25°C, then incubated with ab82645 at a 1/400 dilution for 12 hours at 4°C. The secondary used was a biotinylated goat anti-rabbit polyclonal used at a 1/200.

    See Abreview

  • ab27126 staining HOXA5 in murine endothelial cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed with paraformaldehyde, permeabilized with PBST and then blocked using 1.5% serum for 20 hours at 25°C. Samples were then incubated with ab82645 at a 1/200 dilution for 12 hours at 4°C. The secondary used was an undiluted biotin conjugated goat anti-rabbit polyclonal.

    See Abreview

Anti-HOXA5 antibody (ab82645) 使用論文

This product has been referenced in:
  • Dunn J  et al. Flow-dependent epigenetic DNA methylation regulates endothelial gene expression and atherosclerosis. J Clin Invest 124:3187-99 (2014). WB ; Mouse . Read more (PubMed: 24865430) »
  • Silfa-Mazara F  et al. Oxygen differentially affects the hox proteins Hoxb5 and Hoxa5 altering airway branching and lung vascular formation. J Cell Commun Signal 8:231-44 (2014). WB, IHC ; Mouse . Read more (PubMed: 25073509) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (lung endothelial cells)
Loading amount 20 µg
Specification lung endothelial cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Verified customer

投稿 May 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Endothelial)
Specification Endothelial
Fixative Paraformaldehyde
Permeabilization Yes - PBST
Blocking step Serum as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
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投稿 Nov 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Fetal Lung)
Specification Fetal Lung
Fixative Paraformaldehyde
Permeabilization Yes - PBST
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 1.5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Nov 23 2011

Thank you very much for your interest in ab82645 To our knowledge, ab82645 has not been tested in IHC-Fr on mouse tissue and we do not offer small small/sample size aliquots of antibodies. Therefore, I can offer a discount off a future purchas...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (haematopoietic cell lines)
Loading amount 40 µg
Specification haematopoietic cell lines
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

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Verified customer

投稿 Aug 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"