製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to HOXA4
  • アプリケーション
    適用あり: WBmore details
    適用なし: ICC/IF or IHC-P
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HOXA4.

    (Peptide available as ab32311.)

  • ポジティブ・コントロール
    • HESCS differentiated neuroectoderm lysate at day 10; HESCS differentiated neuroectoderm lysate at day 30.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab26097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37-39 kDa (predicted molecular weight: 34 kDa).
  • 追加情報
    Is unsuitable for ICC/IF or IHC-P.
  • ターゲット情報

    • 機能
      Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5'-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT-3'.
    • 組織特異性
      Embryonic nervous system.
    • 配列類似性
      Belongs to the Antp homeobox family. Deformed subfamily.
      Contains 1 homeobox DNA-binding domain.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Dfd like protein antibody
      • Homeo box A4 antibody
      • Homeobox A4 antibody
      • Homeobox protein Hox-1.4 antibody
      • Homeobox protein Hox-1D antibody
      • Homeobox protein Hox-A4 antibody
      • Hox 1.4 like protein antibody
      • hox-1.4 antibody
      • hox-1d antibody
      • HOX1 antibody
      • HOX1D antibody
      • HOXA4 antibody
      • HXA4_HUMAN antibody
      see all

    画像

    • Lane 1 : Marker
      Lanes 2 - 4 : Anti-HOXA4 antibody (ab26097) at 1 µg/ml

      Lane 1 : As above
      Lane 2 : Neuroectoderm (HESCS differentiated) Lysate - Day 10 at 20 µg
      Lane 3 : Neuroectoderm (HESCS differentiated) Lysate - Day 30 at 20 µg
      Lane 4 : Human Skeletal Muscle Normal Tissue Lysate at 20 µg

      Secondary
      Lanes 2 - 4 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution


      Performed under reducing conditions.

      Predicted band size : 34 kDa
      Observed band size : 30 kDa (why is the actual band size different from the predicted?)

    プロトコール

    参考文献

    This product has been referenced in:
    • Klausen C & Auersperg N HOXA4 protein levels and localization in the aorta and in human abdominal aortic aneurysms. BMC Physiol 11:18 (2011). WB ; Human . Read more (PubMed: 22168796) »
    • Lillvis JH  et al. Regional expression of HOXA4 along the aorta and its potential role in human abdominal aortic aneurysms. BMC Physiol 11:9 (2011). WB, IHC . Read more (PubMed: 21627813) »

    See all 4 Publications for this product

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